Euphorbiaceae, widely known as bellyache bush, is a medicinal plant largely used throughout africa and america. Pdf phytochemical screening and antimicrobial activity of leaf. Leaves and ovary walls contain the maximum contents of phenols, tannins and phytic acid whereas, free amino acids were greater in leaves and seeds fig. The seeds are not edible and contain phorbol ester that is toxic3,4. Physicochemical characterization and phytochemical screening of jatropha.
Scholars research library extraction, physicochemical. In vitro antibacterial activity and phytochemical screening. The present study was carried on methanolic extracts of four jatropha species leaves to investigate the presence of medicinally important phytochemicals in the leaves of different jatropha species. Euphorbiaceae leaves extract vidhya kumari, siddhi gupta, nitu kumari, dr. The phytochemical screening for the methanolic extract revealed the presence of alkaloids, tannins, saponins, flavonoids and cardiac glycosides while the ethanolic anthraquninones and combined anthraquinones. Preliminary phytochemical screening was performed as per standardized procedure of the various phytoconstituents in methanol, chloroform and water extract. The leaf and stem bark was macerated successively in ethanol and aqueous solvent for 24 hrs each. Phytochemical analysis of jatropha gossypifolia linn. Phytochemical investigation of the roots of jatropha curcas l. Krishna kishori verma department of botany, university of rajasthan, jaipur, 302004. The oil from seeds is used in relieving rashes and parasitic skin. Qualitative phytochemistry and antibacterial resistance. Aug 25, 2017 antimicrobial activity and phytochemical screening of stem bark extract of jatropha curcas. Qualitative and quantitative analysis of five secondary metabolites alkaloids, tannins.
Acute and chronic toxicity studies of tubatuba jatropha. Accepted 23 may, 2014 phytochemical studies were carried out on the plant parts of. Jatropha curcas seed collected in june 2008 from banthra research station lucknow, india. In many subtropical and semiarid regions, traditionally, j. Jatropha curcas shows invasive characters and is a significant source of many phytochemicals with varying biological activities. Phytochemical screening was done by standard procedures. Pdf the antimicrobial effects of the methanol, ethyl acetate and hexane extracts of j. Jatropha curcas is a plant that produces high oil content seeds used. Comparative study of the phytochemical properties of jatropha curcas and azadirachta indica plant extracts harryasobara, joy linda and samson, enoobong okon forestry research institute of nigeria, eastern research station, umuahia, abia state, nigeria. Antimicrobial activity and phytochemical screening of stem bark extracts from jatropha curcas linn. Preliminary phytochemical analysis qualitative phytochemical analysis of the crude powder of the leaves collected was determined according to the standard procedures to identify the constituents as described by 1417.
In vitro propagation and phytochemical screening of jatropha curcas. Phytochemical evaluation and antioxidant study of jatropha. Jatropha curcas, nhexane, ethyl acetate, traditional medicine, phytochemical. Physicochemical characterization and phytochemical screening. Phytochemical screening, antimicrobial and antioxidant efficacy of different extracts of rumex dentatus l. The presence of phenolic compounds was identified by taking 1 ml of extract with 5 ml alcohol and a pinch of ferric chloride. Penilaian fitokimia dan biologi biji jatropha curcas nyahlemak. The phytochemical constituents, analgesic and antiinflammatory effects of methanol extract of jatropha curcas leaves in mice and wister albino rats. Phytochemical characterization, antimicrobial activity and reducing potential of seed oil, latex, machine oil and presscake of jatropha curcas amit kumar sharma, mayank gangwar, dharmendra kumar, gopal nath, akhoury sudhir kumar sinha, yamini bhushan tripathi. Jatropha curcas is a species of flowering plant in the spurge family, euphorbiaceae, that is native to the american tropics, most likely mexico and central america. S 1 department of pharmacognosy and ethnotherapy universitty of port harcourt 2 department ofpharmacology university of port harcourt rivers state nigeria. First we studied the phytochemical screening by which we got number of secondary.
Present study investigated the phytochemical components and antimicrobial activities of jatropha caurcas against some selected clinical isolates. Phytochemical analysis and preliminary screening of antimicrobial activity of jatropha curcas l abhijit b shinde abstract in the present study, the antimicrobial activity of jatropha curcas l. The methanolic leaf extract of the plant revealed the presence of bioactive compounds like flavonoids, tannins. In vitro antimicrobial activity and phytochemical screening.
In vitro propagation and phytochemical screening of jatropha curcas shobha gawri, ajay singh, prashant shukla and arunima upadhyay g. Data presented in table 6 showed that the leaves of all studied species contain a significant amount of chlorophyll a. Jatropha curcas is an ornamental plant which is also employed to cure various infections in traditional medicine. The study includes preparation of different extracts by successive solvent extraction for detailed analysis. Phytochemical screening and wound healing activities of. Phytochemical and antibacterial property of crude petroleum ether, chloroform, acetone, methanolic and aqueous extracts of jatropha curcas l leaves were studied as part of searching new bioactive compounds. Phytochemical screening and antimicrobial activities of jatropha curcas l. Issn 22293566 phytochemical screening of leaves of jatropha curcas plant ahirrao r. Acute toxicity studies were done by using female albino mice weighing 2335 gm as. Jatropha caurcas euphorbiaceae is used in folklore medicine to cure various ailments in africa, asia and latin america. Phytochemical and antibacterial property of crude petroleum ether, chloroform, acetone, methanolic and aqueous extracts of jatropha curcas l leaves were studied as part of searching new bioactive. Jatropha curcas, antimicrobial activity, phytochemical screening, minimum inhibitory concentration. Phytochemical analysis of various plant parts of jatropha curcas revealed variation in contents of total phenols, tannins, free amino acids and phytic acid. It is originally native to the tropical areas of the americas from mexico to argentina, and has been spread throughout the world in tropical and subtropical regions around the world, becoming naturalized or invasive in many areas.
Phytochemical studies were carried out on the leaf, stem, root and seed of four jatropha l. Page 117 arkat usa, inc from jatropha gossypifolia two macrocyclic diterpenes, jatrophenone 112 and citlalitrione 1520 were isolated. Euphorbiaceae leaf extract was evaluated against one grampositive species. Acute toxicity studies were done by using female albino mice weighing 2335 gm as per oecd 425 guidelines. Phytochemical profiling of phytoconstituents of grape. Phytochemical evaluation and antioxidant study of jatropha curcas seeds. Phytochemical screening the phytochemical analysis was done according to the method reported by 10,11,12.
In vitro antimicrobial activity and phytochemical screening of jatropha curcas seed extract daniyan s. Phytochemical screening revealed the presence of saponin, steroids, tannin, glycosides, alkaloids and flavonoids in the extracts. The various extract of leaves jatropha curcas linn. Pdf jatropha curcas shows invasive characters and is a significant source of many phytochemicals with varying biological activities. Comparative study of the phytochemical properties of jatropha. The analgesic effect was determined by acetic acid induced writhing test in mice. Phytochemical screening and antimicrobial activities of. Phytochemical screening of the extracts of the root and the latex of jatropha curcas the methods described by odebiyi and sofowora 1978 were used to test for the presence of saponins, tannins, phenolics and alkaloids, lieberman burchad reaction as described by herburne 1973 was used to test for steroids, while the salkowski test was used to. Jatropha curcas euphorbiaceae is a multipurpose plant with many attributes and considerable potential. Antimicrobial activity and phytochemical screening of stem bark extract of jatropha curcas. The in vitro antimicrobial activity of crude ethanolic, methanolic and water extracts of the stem bark of jatropha curcas were investigated. It is a plant that produces seeds with high oil content that can be used as biodiesel. This study investigated the phytoconstituents in neem seed and flower, jatropha curcas stem and root bark and grape stem bark and leaves in some selected solvents.
The uses of various parts of jatropha plant are well known from ages. Pdf phytochemical screening and antimicrobial activity. Pdf in vitro propagation and phytochemical screening of. An value added herbal drug kakade, akshada, magdum, c. The phytochemical screening of the extract was also carried out. The leaves extract of jatropha curcas in chloroform exhibited the presence of alkaloides, cardiac glycosides and steroids. Phytochemical and biological evaluation of defatted seeds. Phytochemical and biological evaluation of defatted seeds of. An value added herbal drug paperback february 6, 2011 by akshada kakade author, c. Experimental section collection of materials jatropha curcas l.
In vitro propagation and phytochemical screening of jatropha. International journal of current microbiology and applied. The preliminary phytochemical screening was carried out in ethyl acetate extract of jatropha gossypifolia by following the method of harbone 9. Jatropha curcas is a species of the genus jatropha, belonging to the family euphorbiaceae. Assessment of phytochemical and antimicrobial screening of. The hexane, ethyl acetate and methanol extracts of this plant were analysed phytochemically and screened against different microorganisms responsible for various infections especially sexually transmitted diseases. The phytochemical constituents, analgesic and anti.
Pharmacognostical and phytochemical evaluation of leaves extract. Phytochemical characterization, antimicrobial activity and reducing. Pdf antimicrobial activity and phytochemical screening of. Jun 03, 2015 phytochemical screening of the extracts of the root and the latex of jatropha curcas the methods described by odebiyi and sofowora 1978 were used to test for the presence of saponins, tannins, phenolics and alkaloids, lieberman burchad reaction as described by herburne 1973 was used to test for steroids, while the salkowski test was used to. Investigation on jatropha curcas yielded a large number of diterpenes table 1. Previous researchers studies different parts of jatropha plant and reported that leaf extract of jatrophacurcas has significant wound healing activity shetty et al. Pdf jatropha is a large genus comprising over 170 species. With this knowledge, the present study was aimed to study the phytochemical and antibacterial property of crude extracts of j. Analysis of the phytochemical content and antimicrobial. Physicochemical characterization and phytochemical screening of.
Leaves and ovary walls were found to contain higher contents of total phenols, tannins and phytic acid whereas free amino acids were greater in leaves. Quantitative phytochemical analysis of selected medicinal plant species by using various organic solvents m madhu, v sailaja, tnvss satyadev, mv satyanarayana abstract. Phytochemical analysis and preliminary screening of. Jatropha curcas, euphorbiaceae, hptlc, pharmacognostical, phytochemical analysis. Physicochemical characterization and phytochemical. In vitro antimicrobial activity and phytochemical analysis. Phytochemical analysis and antimicrobial screening of jatropha curcas were carried out on forest and guinea savannah samples. The analgesic and antiinflammatory effects of the methanolic extract of the leaves of jatropha curcas were investigated in mice and rats respectively. The phytochemical screening of the aqueous, ethanolic and methanolic extracts of the j.
Antimicrobial activity of the biodiesel plant, jatropha. From the determined parameters, jatropha curcas seed oil may be use as cooking oil and in cosmetics. While the anti inflammatory activity was determined by egg albumin induced oedema of the. Pdf phytochemical screening and antimicrobial activity of. Comparative study of the phytochemical properties of.
Rungta college of science and technology, kohkakurud road, bhilai490 024, chhattisgarh, india corresponding author. Pdf physicochemical characterization and phytochemical. The phytochemical screening of the oil showed the presence of saponins, alkaloids, steroids, terpenoids and cardiac glycosides, while tannins, flavonoids and phenols were absent. As a measure of testing the medicinal properties of jatropha curcas, methanol extract obtained from both in vivo leaf and leaf derived callus were subjected to antimicrobial activity against six microorganisms, of the six different concentrations tested, the in vitro leaf callus extracts of at high concentrations 1. Jatropha curcas linn were carried out using standard methods and revealed. Euphorbiaceae leaf extract was evaluated against one grampositive species staphylococcus aureus, and four gramnegative species. Department of microbiology, federal university of technology, minna niger state, nigeria article received on. Several human and veterinary uses in traditional medicine are described for different parts and preparations based on this plant.
Pdf in vitro antimicrobial activity and phytochemical. The minimum inhibitory concentration mic of the ethanol extract was between 0. Phytochemical screening one milliliter of the waterfree and ethanolfree extract solution were tested for the presence of deoxysugars, tannins, alkaloids, sterols, flavonoids, unsaturated lactones. Pdf on jan 10, 2016, m oyama and others published phytochemical screening and antimicrobial activity of leaf extract of jatropha curcas.
The results suggest that the phytochemicals properties of the seed for curing various ailments. In vitro propagation and phytochemical screening of. Antibacterial activity the following bacterial strains escherichia coli, proteus sp. The phytochemical analysis of the methanol extract of the leaf, stem bark and root of. Phytochemical screening revealed the presence of bioactive compound saponin in all the parts of the three plants in water extract only. In vitro antibacterial activity and phytochemical screening of crude extracts from jatropha curcas linn k. The first compound is a novel diterpene while the other was isolated from the species for the first time. In vitro antimicrobial activity and phytochemical analysis of. Phytochemical and biological evaluation of defatted seeds of jatropha curcas penilaian fitokimia dan biologi biji jatropha curcas nyahlemak m uhammad n isar u l h aq, s ultan m ehmood w azir, f aizan u llah, r ahmat a li k han.
The structure of jatrophenone 1 was established mainly by detailed studies of its 1d and 2d nmr. A comparison of pergularia daemia and jatropha curcas leaf extracts. The extracts exhibited antimicrobial activities with zones of inhibition ranging from 5 to 12, 8 to 20 and 0 to 8 mm for ethanol, methanol and water extracts respectively. Comparative phytochemical and antimicrobial screening of.
Pdf on jan 10, 2016, m oyama and others published phytochemical screening and antimicrobial activity of leaf extract of jatropha curcas find, read and cite all the research you need on. Herbal ointment, jatropha curcas, phytochemical screening, wound. Ash value total ash, acid insoluble ash and water soluble ash, extractive values, loss on drying were studied dry weight. Two of them, 2 and 3, are of lathyrane type while the other two, 4 and. Both quantitative and qualitative phytochemical screening was carried out on stems, seeds and leaves of jatropha from forest and guinea savannah. Qualitative result revealed the presence of tannin, saponin, alkaloid, total. Phytochemical and antibacterial studies on jatropha curca s l. Phytochemistry and antimicrobial assay of jatropha curcas extracts. Antimicrobial activity and phytochemical screening of stem. Qualitative phytochemical screening of jatrophacurcas extract. Different plant parts of jatropha curcas l exhibited variation in their phytochemical constituents.
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